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human skin cancer cell line a431  (ATCC)


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    Structured Review

    ATCC human skin cancer cell line a431
    Human Skin Cancer Cell Line A431, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 3745 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/skin+cancer+cell+a431/A-431/pm40536708-205-0-31
    Average 98 stars, based on 3745 article reviews
    human skin cancer cell line a431 - by Bioz Stars, 2026-09
    98/100 stars

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    Cell Culture:

    Article Title: Aminopyrimidine compound used for inhibiting activity of protein kinase
    Article Snippet: .. Cell lines: skin cancer cell A431 (purchased from the American Type Culture Collection (ATCC)); lung cancer cell NCI-H1975 (purchased from the American Type Culture Collection (ATCC)) and HCC827 (purchased from the American Type Culture Collection (ATCC)); all were cultured with RPM11640 medium containing 10% fetal bovine serum, 100 U/ml penicillin, and 100 μg/nl streptomycin. .. Reagents and materials: RPMI-1640 (GIBCO, Cat. No A10491-01); fetal bovine serum (GIBCO, Cat. No 10099141): 0.25% trypsin-EDTA (GIBCO, Cat. No 25200); penicillin-streptomycin; liquid (GIBCO, Cat. No 15140-122); DMSO (Sigma, Cat. No D2650); MTS Assay Kit (Promega, Cat. No G3581), 96-well plate (Corning, Cat. No 3365).



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    ATCC skin cancer cells a431
    Figure 3. EGFR as a direct target of miR-218-5p. (A) The 3′-UTR region of EGFR to which miR-218-5p binds. (B) Luciferase reporter constructs containing wild-type (WT) and EGFR 3′-UTR mutant (MUT) plasmid. (C) Relative luciferase activities measured with miR-NC or miR-218-5p overexpression using HEK-293 T cells. Renilla luciferase reporter was used as an internal control. Data were represented by means ±SE. (D) As-T, Cr-T, and <t>A431</t> cells transfected with miR-NC or miR-218-5p mimic, and the EGFR and β-actin protein expression measured using immunoblotting assay. * p < 0.05; *** p < 0.001; ns, not significant. The original western blots of this figure is Figure S7.
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    Figure 3. EGFR as a direct target of miR-218-5p. (A) The 3′-UTR region of EGFR to which miR-218-5p binds. (B) Luciferase reporter constructs containing wild-type (WT) and EGFR 3′-UTR mutant (MUT) plasmid. (C) Relative luciferase activities measured with miR-NC or miR-218-5p overexpression using HEK-293 T cells. Renilla luciferase reporter was used as an internal control. Data were represented by means ±SE. (D) As-T, Cr-T, and A431 cells transfected with miR-NC or miR-218-5p mimic, and the EGFR and β-actin protein expression measured using immunoblotting assay. * p < 0.05; *** p < 0.001; ns, not significant. The original western blots of this figure is Figure S7.

    Journal: Cancers

    Article Title: MiR-218-5p/EGFR Signaling in Arsenic-Induced Carcinogenesis.

    doi: 10.3390/cancers15041204

    Figure Lengend Snippet: Figure 3. EGFR as a direct target of miR-218-5p. (A) The 3′-UTR region of EGFR to which miR-218-5p binds. (B) Luciferase reporter constructs containing wild-type (WT) and EGFR 3′-UTR mutant (MUT) plasmid. (C) Relative luciferase activities measured with miR-NC or miR-218-5p overexpression using HEK-293 T cells. Renilla luciferase reporter was used as an internal control. Data were represented by means ±SE. (D) As-T, Cr-T, and A431 cells transfected with miR-NC or miR-218-5p mimic, and the EGFR and β-actin protein expression measured using immunoblotting assay. * p < 0.05; *** p < 0.001; ns, not significant. The original western blots of this figure is Figure S7.

    Article Snippet: Skin cancer cells A431 were also purchased from ATCC (Manassas, VA, USA).

    Techniques: Luciferase, Construct, Mutagenesis, Plasmid Preparation, Over Expression, Control, Transfection, Expressing, Western Blot